"To prove that tuberculosis is caused by the invasion of bacilli, and that it is a parasitic disease primarily caused by the growth and multiplication of bacilli, it is necessary to isolate the bacilli from the body, to grow them in pure culture until they are freed from every disease product of the animal organism, and, by introducing isolated bacilli into animals, to reproduce the same morbid condition that is known to follow from inoculation with spontaneously developed tuberculous material."

Source : Robert Koch (1987). “Essays of Robert Koch”, Praeger Pub Text

Robert Koch

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